DESCRIPTION:
Leading experts distill their extensive experience into user-friendly methods for the study of fluid-electrolyte homeostasis and kidney function in health and disease. Described in step-by-step detail, the techniques move from the molecular level to the whole organism, from simple models to integrative physiology. Here the researcher will find established disease models, methods for optimizing renal disease research, as well as much help in choosing imaging techniques for studies of the kidney""s structure and function in health, disease, and during embryonic development. Subjects covered include molecular diagnostics, strategies for studying the molecular mechanisms of kidney disease, technical means to assess the functional correlates of disease, and the planning of clinical trials.
CONTENTS:
PART I. OPTIMIZING THE USAGE OF MODELS OF RENAL DISEASE
1 Standards of Animal Care in Biological Experiments
2 Models of Polycystic Kidney Disease
3 Rat Models of the Metabolic Syndrome
4 Models of Glomerulonephritis
PART II. CHOICES OF IMAGING TECHNIQUES IN STUDIES OF RENAL DISEASE
5 Functional Studies of the Kidney with Magnetic Resonance Imaging
6 Use of Radionuclides to Study Renal Function
7 Intravital Videomicroscopy
8 Confocal and Two-Photon Microscopy
9 Atomic Force Microscopy in Renal Physiology
10 Freeze-Fracture Analysis of Renal-Epithelial Tight Junctions
PART III. STUDIES OF RENAL DEVELOPMENT
11 Organ Culture of Intact Metanephric Kidneys
12 The Ureteric Bud: Tissue-Culture Approaches to Branching Morphogenesis and Inductive Signaling
13 Studies of Cell Lineage in the Developing Kidney
14 Transient Transfection Assays for Analysis of Signal Transduction in Renal Cells
PART IV. APPROACHES TO STUDY MOLECULAR MECHANISMS OF DISEASE
15 The Study of Gene Polymorphisms: How Complex Is Complex Genetic Disease?
16 Laser-Capture Microdissection
17 Serial Analysis of Gene Expression
18 RNA Labeling and Hybridization of DNA Microarrays
19 Gene-Expression Analysis of Microdissected Renal Biopsies
20 The Use of SELDI ProteinChip® Array Technology in Renal Disease Research
PART V. TECHNICAL MEANS TO ASSESS FUNCTIONAL CORRELATES OF DISEASE
21 Clearance Studies in Genetically Altered Mice
22 Long-Term Blood-Pressure Monitoring in Unrestrained Animals
23 In Vitro Studies on Renin Release
24 Mitochondrial Function
25 Detection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney Proteins
26 Products of Arachidonic Acid Metabolism
27 Methods for Measurements of Heme Oxygenase (HO) Isoforms-Mediated Synthesis of Carbon Monoxide and HO-1 and HO-2 Proteins
28 The Juxtamedullary Nephron Preparation
29 Tubuloglomerular Feedback
30 Microdissected Perfused Vessels
31 Renal Microperfusion Techniques
32 How to Design a Clinical Trial
