carrito Compra

(0 Productos)

Gastos de envío GRATIS

Solo para España


MUSCULAR DYSTROPHY‚ Methods and Protocols

MUSCULAR DYSTROPHY- Methods and Protocols-9780896036956
  1. Autor:

    Bushby

  2. Isbn:

    9780896036956

  3. Páginas:

    300

  4. Año:

    2000

  5. Edición:

    1

  6. Idioma:

    Ingles

  7. Disponibilidad:

    De 2 a 3 Semanas

Precio s/iva:

88.00 €

83,60  €
Volver

DESCRIPTION:
Katherine Bushby and Louise Anderson assemble an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy. Each method is highly detailed to ensure success and is presented by a hands-on expert. The various DNA techniques focus on both the X-linked muscular dystrophies and the autosomal recessive muscular dystrophies. The protein methods include expression analysis, multiplex western blot analysis, immunocytochemical analysis, and reviews of immunological reagents and of amplification systems. Comprehensive and highly practical, Muscular Dystrophy: Methods and Protocols offers today""s diagnostic laboratories, basic and medical researchers, and clinicians an authoritative collection of tools that will serve as exacting diagnostic tools as well as greatly empowering research on the novel therapeutics now beginning to emerge.

CONTENTS:
PART I. BACKGROUND
1. Application of Molecular Methodologies in Muscular Dystrophies
2. Clinical Examination as a Tool for Diagnosis: Historical Perspective
3. Histopathological Diagnosis of Muscular Dystrophies
4. Serum Creatine Kinase in Progressive Muscular Dystrophies

PART II. THE MOLECULAR APPROACH
A. GENETICS: X-Linked Muscular Dystrophy
5. Deletion and Duplication Analysis in Males Affected with Duchenne or Becker Muscular Dystrophy
6. Point Mutation Detection in the Dystrophin Gene
7. DNA-Based Techniques for Detection of Carriers of Duchenne and Becker Muscular Dystrophy
8. Fluorescence In Situ Hybridization Analysis for Carrier Detection in Duchenne/Becker Muscular Dystrophy
9. DNA-Based Prenatal Diagnosis for Duchenne and Becker Muscular Dystrophy
10. Molecular Diagnosis and Genetic Counseling of the Manifesting Carrier of Duchenne Muscular Dystrophy
11. Mutation Analysis of X-Linked Emery-Dreifuss Muscular Dystrophy Gene

B. GENETICS: Autosomal Recessive Muscular Dystrophies
12. Analysis of the LAMA2 Gene in Merosin-Deficient Congenital Dystrophy
13. ƒ¿-Sarcoglycan Mutations
14. Mutation Detection in ƒÀ- and ƒÁ-Sarcoglycan (LGMD2E and LGMD2C)
15. Mutation Analysis in ƒÂ-Sarcoglycan (LGMD2F)
16. Molecular Diagnosis of Calpainopathies: Methods Used for Detection of Mutations in CAPN3 Gene Implicated in Limb-Girdle Muscular Dystrophy Type 2A
17. Molecular Investigation of LGMD2B-Haplotype Analysis and Mutation Screening
18. Molecular Analysis of Facioscapulohumeral Muscular Dystrophy (FSHD1)

III. PROTEIN ANALYSIS IN THE MUSCULAR DYSTROPHIES
19. Analysis of Protein Expression in Muscular Dystrophies
20. Immunological Reagents and Amplification Systems
21. Immunocytochemical Analysis
22. Multiplex Western Blot Analysis of Muscular Dystrophy Proteins
23. Fetal Muscle Biopsy

PART IV. FUTURE PERSPECTIVES
24. Use of Animal Models to Understand Human Muscular Dystrophy
25. Options for Development of Gene-Based Therapy for Muscular Dystrophy



Libreria Médica Berri 2024 ®