DESCRIPTION:
Studying plant biology at the molecular level requires different techniques from those used in the study of animals and bacteria because of the different structure of plant cells and the different mechanisms they employ. The current interest in crop research and the genetic modification of food plants means it is an expanding discipline, and the editors have assembled distinguished contributors to give it comprehensive coverage. Volume 2 is focused on gene expression and gene product analysis.
CONTENTS:
Gene Expression
1. Transcript Analysis
2. In situ hybridization
3. DNA micro-arrays for gene expression analysis
4. DNA-protein interactions
5. Inducible gene expression in plants
Gene Product Analysis
6. Expression of recombinant proteins
7. Import of proteins into isolated chloroplasts and thylakoid membranes
8. Fractionation of plant tissue for biochemical analyses
9. Studying protein-protein interactions with the yeast two-hybrid system
10. Antibody Techniques
11. Antibody phage display libraries
Functional Analysis In Vivo
12. Spatial and temporal measurements of calcium ions in living plant cells
13. Reporter genes and in vivo imaging
14. Moss gene technology
A1. List of suppliers
