DESCRIPTION:
Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose and then goes into detailed protocols for every step of analysis. Several chapters also include a section with tips on individual steps not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting.
Among the topics discussed in this volume are Staphylococcus aureus infections, MIC analysis, ITS-PCR identification, SCCmec analysis, and Biofilm formation. The book also contains helpful case studies, and authors provide insights into the control, prevention, and treatment of infections.
Oriented towards routine clinical diagnosis, research, and actual practice for treatment of patients infected by MRSA, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols will provide a useful basis for anyone interested in instituting a new method for studying MRSA.
CONTENTS:
Chapter 1. Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections
Chapter 2. MRSA Case Studies
Chapter 3. MIC analysis and susceptibility testing of MRSA
Chapter 4. ITS-PCR identification of MRSA
Chapter 5. Pulsed-field Gel Electrophoresis of Methicillin-resistant Staphylococcus aureus
Chapter 6. Multilocus sequence typing (MLST) of Staphylococcus aureus
Chapter 7. Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA
Chapter 8. Targeted gene disruption for the analysis of virulence of Staphylococcus aureus
Chapter 9. Molecular Analysis of Staphylococcal Superantigens
Chapter 10. Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus
Chapter 11. Comparative analysis of staphylococcal adhesion and internalization by epithelial cells
Chapter 12. Comparative analysis of MRSA
Chapter 13. Genomic analysis of gene expression of Staphylococcus aureus
Chapter 14. Proteomic approach to investigate Methicillin-Resistant Staphylococcus aureus (MRSA)
Chapter 15. Environmental surveillance for methicillin-resistant Staphylococcus aureus
Chapter 16. The Control and Prevention of MRSA Infections
Chapter 17. Treatment of Infections Due to Resistant Staphylococcus aureus
