DESCRIPTION:
Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular BiologyÖ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.
CONTENTS:
1. Digital Images Are Data: And Should Be Treated as Such
2. Epi-Fluorescence Microscopy
3. Live-Cell Migration and Adhesion Turnover Assays
4. Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic Proteins in Human Venous Valves
5. Colocalization Analysis in Fluorescence Microscopy
6. A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown
7. Light Sheet Microscopy in Cell Biology
8. Image-Based High-Throughput Screening for Inhibitors of Angiogenesis
9. Intravital Microscopy to Image Membrane Trafficking in Live Rats
10. Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential Interference Contrast Microscopy and Planar Illumination Microscopy
11. Laser Scanning Cytometry: Principles and Applications_An Update
12. Laser Capture Microdissection for Protein and NanoString RNA Analysis
13. Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy
14. Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy
15. Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation
16. Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells
17. Porosome: The Secretory NanoMachine in Cells
18. Stereology and Morphometry of Lung Tissue
19. A Novel Combined Imaging/Morphometrical Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis
20. Correlative Light-Electron Microscopy as a Tool to Study In Vivo Dynamics and Ultrastructure of Intracellular Structures
21. Photooxidation Technology for Correlative Light and Electron Microscopy
22. Electron Microscopy of Endocytic Pathways
23. Morphological Analysis of Autophagy
24. Cytochemical Detection of Peroxisomes and Mitochondria
25. Histochemical Detection of Lipid Droplets in Cultured Cells
26. Environmental Scanning Electron Microscopy in Cell Biology
27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology
28. High-Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles
29. ADI Imaging Mass Spectrometry for Direct Tissue Analysis
