DESCRIPTION:
A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues.
This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical guidance on the collection, sampling and analysis of mouse tissue samples in order to maximize the information that can be gained from these tissues. As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally- induced lesions.
This will be an essential bench-side companion for researchers and practitioners looking for an accessible and well-illustrated guide to mouse pathology.
- Written by experienced pathologists and specifically tailored to the needs of scientists and histologists
- Full colour throughout
- Provides advice on sampling tissues, necropsy and recording data
- Includes common anatomic variations, background lesions and artefacts which will help non-experts understand whether histologic variations seen are part of the normal background or related to their experimental manipulation
CONTENTS:
Chapter 1 Necropsy of the mouse
1.1 Recording of findings
1.2 Bleeding technique
1.3 Perfusion
1.4 External examination
1.5 Weighing of organs
1.6 Positioning of mouse for necropsy and removing the skin
1.7 Opening the abdominal cavity and exposing organs
1.8 Removing the ribcage to expose lungs and heart
1.9 Removing the brain and spinal cord
1.10 Collecting and fixing tissue samples
Chapter 2 Practical approaches to reviewing and recording pathology data
2.1 Sample selection and trimming patterns
2.2 Controls
2.3 Standardizing terminology
2.4 Microscopic terminology
2.5 Recording pathology data
2.6 Quantitative versus semiquantitative analysis
2.7 Semiquantitative techniques
2.8 Quantitative techniques
Chapter 3 Gastrointestinal system
3.1 Background and development
3.2 Oral cavity
3.3 Salivary glands
3.4 Stomach and intestines
3.5 Liver
3.6 Pancreas
Chapter 4 Cardiovascular system
4.1 Background and development
4.2 Sampling techniques and morphometry
4.3 Artefacts
4.4 Anatomy and histology of the heart
4.5 Anatomy and histology of the blood vessels
Chapter 5 Urinary system
5.1 Background and development
5.2 Sampling techniques
5.3 Artefacts
5.4 Background lesions
5.5 Anatomy and histology
Chapter 6 Reproductive system
6.1 Background and development of the male and female reproductive tract
6.2 Female reproductive tract
6.3 Male reproductive tract
Chapter 7 Endocrine system
7.1 Introduction
7.2 Adrenals
7.3 Pituitary
7.4 Thyroids and parathyroids
Chapter 8 Nervous system
8.1 Introduction
8.2 Necropsy
8.3 Fixation/perfusion
8.4 Trimming
8.5 Special stains and techniques
8.6 Microscopic examination
8.7 Normal histology-juvenile - CNS and PNS
8.8 Artefacts - CNS and PNS
8.9 Pathological changes and background pathology - CNS and PNS
8.10 Acknowledgment
Chapter 9 Lymphoid and haematopoietic system
9.1 Introduction
9.2 Lymph nodes
9.3 Spleen
9.4 MALT (mucosa-associated lymphoid tissue)
9.5 Thymus
9.6 Bone marrow
Chapter 10 Integument and adipose tissue
10.1 Background and development
10.2 Sampling technique
10.3 Anatomy and histology
Chapter 11 The respiratory system
11.1 Background and development
11.2 Embryology
11.3 Anatomy and histology of the respiratory system
11.4 Upper respiratory tract
11.5 Lower respiratory tract
Chapter 12 Special senses
12.1 Background and development
12.2 Sampling technique for the eye
12.3 Artefacts
12.4 Anatomy and histology of the eye and associated glands
12.5 Background and development of the ear
12.6 Sampling technique for the ear and associated structures
12.7 Anatomy and histology of the ear and associated glands
Chapter 13 Musculoskeletal system
13.1 Background and development
13.2 Sampling techniques
13.3 Anatomy and histology
